Applications of Differential-Display Reverse Transcription-PCR to Molecular Pathogenesis and Medical Mycology

Applications of differential-display reverse transcription-PCR to molecular pathogenesis and medical mycology.

The host-fungus interaction is characterized by changes in gene expression in both host and pathogen. Differential-display reverse transcription PCR (DDRT-PCR) is a PCR-based method that allows extensive analysis of gene expression among several cell populations. Several limitations and drawbacks to this procedure have now been addressed, including the large number of false-positive results and...

Identification of stress-responsive genes in Streptococcus mutans by differential display reverse transcription-PCR.

Streptococcus mutans, which causes dental caries in the human oral cavity and occasionally causes infective endocarditis in the heart, withstands adverse environmental stress through diverse alterations in protein synthesis. Differential gene expression in response to environmental stress was analyzed by RNA fingerprinting using arbitrarily primed PCR with a panel of 11mer primers designed for ...

One-Step Reverse Transcription PCR (RT-PCR). Fatemeh Mehrpooyan*

Reverse transcription-PCR differential display analysis of Escherichia coli global gene regulation in response to heat shock.

A reverse transcription (RT)-PCR technique was developed to analyze global gene regulation in Escherichia coli. A novel combination of primers designed specifically for the start and stop regions of E. coli genes (based on the findings of Fislage et al. [R. Fislage, M. Berceanu, Y. Humboldt, M. Wendt, and H. Oberender, Nucleic Acids Res. 25:1830-1835, 1997]) was used as an alternative to the po...

Microbial Cell Surface Display: Its Medical and Environmental Applications

Cell-surface display is the expression of peptides and proteins on the surface of living cells by fusing them tofunctional components of cells which are exposed to the environment of cells. This strategy can be carriedout using different surface proteins of cells as anchoring motifs and different proteins from different sourcesas a passenger protein. It is a promising strategy...